WB result of CD70 Recombinant Rabbit mAb
Primary antibody: CD70 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: PC-3 whole cell lysate 20 µg
Lane 4: U266B1 whole cell lysate 20 µg
Lane 5: SK-OV-3 whole cell lysate 20 µg
Low expression control: Jurkat whole cell lysate; SH-SY5Y whole cell lysate; PC-3 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 25, 54, 85 kDa
CD70 Recombinant Rabbit mAb (S-1725-277)
CD70 Recombinant Rabbit mAb (S-1725-277)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD70 |
| Synonyms | CD70 antigen; CD27 ligand (CD27-L); Tumor necrosis factor ligand superfamily member 7Imported; CD27L; CD27LG; TNFSF7 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P32970 |
| Clone Number | S-1725-277 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | U266B1, SK-OV-3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:100 | Hu |
Background
CD70, also known as CD27 ligand, is a type Ⅱ transmembrane protein that plays a crucial role in the immune system. It is primarily expressed on antigen - presenting cells (APCs) such as dendritic cells, macrophages, and B cells, as well as on activated T cells. CD70 interacts with its receptor CD27, which is mainly found on T cells, B cells, and natural killer (NK) cells. This interaction provides important co - stimulatory signals that promote T cell activation, proliferation, and differentiation, and also enhances B cell and NK cell functions. Additionally, CD70 is involved in the regulation of immune responses, including the maintenance of immune homeostasis and the induction of immune - mediated tumor cell killing. Abnormal expression of CD70 has been associated with various diseases, such as autoimmune disorders and certain types of cancers. Therefore, CD70 is considered a promising target for the development of immunotherapies.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adneocarcinoma. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD70 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U266B1 cells (top panel) and negative staining in Jurkat cells (below panel). Anti-CD70 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
