WB result of CD68 Recombinant Rabbit mAb
Primary antibody: CD68 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: J774A.1 whole cell lysate 20 µg
Lane 3: RAW264.7 whole cell lysate 20 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 110 kDa
CD68 Recombinant Rabbit mAb (S-3394)
CD68 Recombinant Rabbit mAb (S-3394)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD68 |
| Synonyms | Macrosialin; Gp110 |
| Location | Cell membrane |
| Accession | P34810 |
| Clone Number | S-3394 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Ms, Rt |
| Positive Sample | J774A.1, RAW264.7, mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms |
| IHC-P | 1:250 | Ms, Rt |
| ICC | 1:500 | Ms |
| IF | 1:500 | Ms, Rt |
Background
CD68 is a heavily glycosylated, 110 kDa type I transmembrane glycoprotein of the lysosome-associated membrane protein (LAMP) family (also called LAMP-4) that is highly expressed on human monocytes, macrophages, osteoclasts, and other myeloid cells, where it predominantly localizes to lysosomes and endosomes and participates in phagocytosis, debris clearance, and inflammatory responses via its scavenger-receptor activity, while alternative splicing produces multiple isoforms and its presence on tumor cells and ability to bind selectins implicate it in metastasis, making it a widely used histological macrophage marker.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD68 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD68 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-CD68 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-CD68 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in J774A.1 cells. Anti-CD68 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse spleen. Anti-CD68 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat spleen. Anti-CD68 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
