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CD66b Nanobeads, human（RUO）

CD66b Nanobeads, human（RUO）

Catalog Number: S0K0023 Reactivity: Human Conjugation: Brand: Starter
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Product Details

Product Specification


Format

Dextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD66b monoclonal antibody
Capacity

1 mL magnetic beads: suitable for a total cell count of 5 × 108and can perform up to 50 sorting runs.

2 mL magnetic beads: suitable for a total cell count of 1 × 109and can perform up to 100 sorting runs.
Principle of Separation

CD66b Nanobeads are used to magnetically label human CD66b⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD66b⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD66b⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD66b⁺ cells can be eluted, yielding the positive sorted fraction.
Applications in cell sorting Isolate or deplete CD66b+ cells from human whole blood (following red blood cell lysis).
Reactivity Human
Magnetic Bead Size 20 nm
Separation Method Column-Based
Selection Strategy Positive Selection
Stability & Storage

Store protected from light at 2–8°C; do not freeze.

Background

CD66b is a glycosylphosphatidylinositol (GPI)-anchored protein with a molecular weight of 95–100 kDa, also known as CD67, CGM6, and NCA-95. This molecule is expressed on the surface of neutrophils and eosinophils but is not expressed on basophils or lymphocytes.

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Validation Data

Use CD66b Nanobeads, a human S or L Separation Column, and a magnetic separator to enrich or deplete CD66b+ cells from human whole blood. Subsequently, perform fluorescent staining with Alexa Fluor® 488 Mouse Anti-Human CD66b Antibody (G10F5) (STD Cat: S0B8109) and APC anti-human CD14 Antibody (Clone: HCD14), and gate on the live cell population for analysis.

DLS

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