CD64 Recombinant Mouse mAb (S-5504)
CD64 Recombinant Mouse mAb (S-5504)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD64 |
| Synonyms | High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI (FcRI); Fc-gamma RIA (FcgammaRIa); CD64; FCG1; FCGR1; IGFR1; FCGR1A |
| Location | Cell membrane |
| Accession | P12314 |
| Clone Number | S-5504 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Predicted Reactivity | AfGrMk |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
Background
CD64 is a single-pass transmembrane glycoprotein of the immunoglobulin superfamily that forms the ligand-binding backbone of the tetrameric high-affinity IgE receptor (FcεRI) expressed on mast cells and basophils; its extracellular region folds into two Ig-like domains that clamp the Fc portion of IgE with picomolar affinity, positioning the constant heavy-chain ε2-3 interface for optimal recognition, while a short cytoplasmic tail lacks signaling motifs but critically nucleates the entire receptor complex by juxtamembrane interactions with the four-helix FcεRIβ subunit and the disulfide-linked FcRγ homodimer, thereby dictating the architecture of the transmembrane three-helix bundle that propagates ITAM-based phosphorylation cascades upon IgE/allergen crosslinking and drives immediate degranulation, cytokine release and the clinical manifestations of type I hypersensitivity.
Picture
Picture
Western Blot
WB result of CD64 Recombinant Mouse mAb
Primary antibody: CD64 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 60-75, 38-43 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD64 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-CD64 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CD64 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD64 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
