WB result of CD56 Recombinant Rabbit mAb
Primary antibody: CD56 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: NK-92 whole cell lysate 20 µg
Lane 3: SH-SY5Y whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 95 kDa
Observed MW: 140-300 kDa
This blot was developed with high sensitivity substrate
CD56 Recombinant Rabbit mAb (SDT-2427-1)
CD56 Recombinant Rabbit mAb (SDT-2427-1)
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Product Details
Product Details
Product Specification
| Antigen | CD56 |
| Synonyms | Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; NCAM; NCAM1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P13591 |
| Clone Number | SDT-2427-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | NK-92, SH-SY5Y |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
Neural cell adhesion molecule 1 (NCAM-1), also known as CD56, is a cell adhesion protein belonging to the immunoglobulin superfamily. It is expressed on various cell types including neurons, muscle cells, and immune cells like natural killer (NK) cells. NCAM-1/CD56 plays crucial roles in cell-to-cell and cell-matrix interactions during development and differentiation, regulating processes such as neurogenesis, neurite outgrowth, axonal guidance, and cell migration in the nervous system. In the immune system, it is involved in the expansion of T lymphocytes, B lymphocytes, and NK cells. NCAM-1/CD56 also triggers signaling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). Additionally, it serves as a biomarker for certain diseases, such as malignant tumors in the nervous system and neuroendocrine carcinoma.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD56 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CD56 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CD56 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human NK/T-cell lymphoma. Anti-CD56 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in SH-SY5Y cells (top panel) and negative staining in MCF7 cells (below panel). Anti-CD56 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
