CD56+ cells are enriched from or depleted of human peripheral blood mononuclear cells (PBMCs). Subsequently, fluorescent staining is performed using FITC-conjugated mouse anti-human CD3 antibody (UCHT1) and APC-conjugated anti-human CD56 (NCAM) antibody (clone: W22097A), and the live cell population is gated for analysis.
CD56 Nanobeads, human（RUO）
CD56 Nanobeads, human（RUO）
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD56 monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations 1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 separations |
| Principle of Separation | CD56⁺ cells are magnetically labeled using CD56 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD56⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD56⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD56⁺ cells can be eluted, yielding the positive sorted fraction. |
| Reactivity | Human |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD56 Nanobeads, humanIt is designed to isolate human cells based on the expression of the CD56 antigen. CD56 is predominantly expressed on the surface of all human NK cells, and its density on the cell membrane increases upon activation. This antigen is also present in a distinct population of CD3-positive cells.+On T cells (mediating MHC‑unrestricted cytotoxicity), as well as on myoblasts, certain neural tissues, and tumors.
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Validation Data
