CD56 Nanobeads, human（RUO）

Catalog Number: S0K0019 Reactivity: Human Conjugation: Brand: Starter

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$417 USD

Size: 1 mL 2 mL

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Product Details

Product Specification

Format	Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD56 monoclonal antibody
Capacity	2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations 1 mL magnetic beads: suitable for 5×10⁸total cells, and can perform up to 50 separations
Principle of Separation	CD56⁺ cells are magnetically labeled using CD56 Nanobeads for humans. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD56⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD56⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD56⁺ cells can be eluted, yielding the positive sorted fraction.
Reactivity	Human
Magnetic Bead Size	20 nm
Separation Method	Column-Based
Selection Strategy	Positive Selection
Stability & Storage	Store protected from light at 2–8°C; do not freeze.

Background

CD56 Nanobeads, humanIt is designed to isolate human cells based on the expression of the CD56 antigen. CD56 is predominantly expressed on the surface of all human NK cells, and its density on the cell membrane increases upon activation. This antigen is also present in a distinct population of CD3-positive cells.⁺On T cells (mediating MHC‑unrestricted cytotoxicity), as well as on myoblasts, certain neural tissues, and tumors.

Protocol

Note: This kit does not include the sorting buffer; please prepare it yourself. The corresponding Stretta catalog number isS0D3018

Taking S as an exampleSeparation Columnas an example

	Steps	Operating procedures	Dosage / Time
Magnetic labeling	1	Count the total number of cells	—
	2	Centrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant	400×g, 7 min
	3	Add 80 μL of buffer to resuspend every 1×10⁷ cells Note:Adjust the volume of buffer proportionally according to the sample volume	Resuspend the cells
	4	Add 20 μL per 1×10⁷ cellsCD56 Nanobeads, human Note:When the cell quantity increases, increase the volume of all reagents proportionally	Add 20 μL per 1×10⁷ cells
	5	Mix thoroughly, and incubate in the dark at 2–8℃ for 15 minutes	Incubate for 15 min
	6	Wash every 1×10⁷ cells with 1 mL of buffer, centrifuge at 400×g for 7 minutes, and completely discard the supernatant.	Add 1 mL to 1×10⁷ cells
	7	Resuspend up to 1×10 in 500 μL of buffer⁸Cells	Resuspend cells
Magnetic separation	8	Place the S-type separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 500 μL of buffer.	Rinse the separation column
	9	Load the cell suspension onto the separation column and collect the effluent (unlabeled cells).	—
	10	Wash the column with buffer three times, 500 μL each time, and add the next wash only after the liquid inside the column has completely drained.	3 × 500 μL of buffer
	11	Combine all effluents into the negative fraction; remove the separation column from the magnetic field and place it over the collection tube.	—
	12	Add 1 mL of buffer to the column, and quickly and firmly push the accompanying plunger to elute the magnetically labeled positive cells. Note:For higher purity, use a new column and repeat the separation once.	Collect the target cells.

With LSeparation Columnas an example.

	Steps	Operating method	Dosage / Time
Magnetic labeling	1	Count the total number of cells	—
	2	Centrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant.	400×g, 7 min
	3	Resuspend every 1×10⁷ cells in 80 μL of buffer. Note:Adjust the volume of the buffer proportionally according to the sample volume.	Resuspend the cells.
	4	Add 20 μL per 1×10⁷ cells.CD56 Nanobeads, human. Note:When the cell quantity increases, increase the volume of all reagents proportionally.	Add 20 μL per 1×10⁷ cells.
	5	Mix thoroughly and incubate in the dark at 2–8℃ for 15 minutes.	Incubate for 15 min
	6	Add 1 mL of buffer to wash every 1×10⁷ cells, centrifuge at 400×g for 7 minutes, and completely aspirate the supernatant.	Add 1 mL to 1×10⁷ cells
	7	Resuspend up to 1×10 in 500 μL of buffer⁸cells	Resuspend the cells
Magnetic separation	8	Place the L-shaped separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 3 mL of buffer.	Rinse the separation column
	9	Load the cell suspension onto the separation column and collect the effluent (unlabeled cells).	—
	10	Wash the column with buffer three times, 3 mL each time, and add the next wash only after the liquid inside the column has completely drained.	3 × 3 mL of buffer
	11	Combine all effluents into the negative fraction; remove the separation column from the magnetic field and place it over the collection tube.	—
	12	Add 5 mL of buffer to the column, and quickly and firmly push the accompanying plunger to elute the magnetically labeled positive cells. Note:For higher purity, use a new column and repeat the separation once.	Collect the target cells.

Picture

Validation Data

CD56+ cells are enriched from or depleted of human peripheral blood mononuclear cells (PBMCs). Subsequently, fluorescent staining is performed using FITC-conjugated mouse anti-human CD3 antibody (UCHT1) and APC-conjugated anti-human CD56 (NCAM) antibody (clone: W22097A), and the live cell population is gated for analysis.

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CD56 Nanobeads, human（RUO）