1 4

CD5(159-372) Fc Chimera Protein, Human

CD5(159-372) Fc Chimera Protein, Human

Catalog Number: UA016083 Brand: UA BIOSCIENCE
Share:
Price:
$215 USD
$215 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms CD5, LEU1, T1, Tp67
Accession P06127
Amino Acid Sequence

Leu 159 - Pro 372 with hIgG1 Fc Tag at the C-Terminus
Expression System HEK293
Molecular Weight

55-70kDa (Reducing)

Purity ＞95% by SDS-PAGE & HPLC
Conjugation Unconjugated
Tag Human IgG1 Fc
Physical Appearance Lyophilized powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. Van de Velde, H., et al. (1991). The B-cell surface protein CD72/Lyb-2 is the ligand for CD5. Nature, 351(6328), 662-665.
2. McAlister, M.S., et al. (1998). Structural analysis of the CD5 antigen--expression, disulphide bond analysis and physical characterisation of CD5 scavenger receptor superfamily domain 1.
3. Garza-Garcia, A., et al. (2008). Three-dimensional solution structure and conformational plasticity of the N-terminal scavenger receptor cysteine-rich domain of human CD5. Journal of Molecular Biology, 378(1), 129-144.

Background

CD5 is a type I transmembrane glycoprotein with a molecular weight of approximately 67 kDa. Its extracellular region is composed of three group B scavenger receptor cysteine-rich domains (D1, D2, D3), each containing 8 cysteines. The cytoplasmic domain comprises 93 amino acids and includes an immunoreceptor tyrosine-based inhibitory motif-like domain responsible for recruiting the protein tyrosine phosphatase SHP-1.

The 159-372 amino acid fragment of the human CD5 protein corresponds to the second and third SRCR domains of its extracellular region. This fragment is typically located in the membrane-proximal region of full-length CD5. By lacking the N-terminal first SRCR domain, its potential conformation and ligand-binding properties may differ from the full extracellular region, making it an important target for functional studies.

This fragment contains multiple functional sites and may be involved in interactions with other immune molecules, such as CD72 on the surface of B cells. Due to the high conservation of SRCR domains, this fragment likely plays a crucial role in maintaining the structural integrity of the whole protein.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of CD5(159-372) Fc Chimera Protein, Human is more than 95% determined by SEC-HPLC.

RP-HPLC

The purity of CD5(159-372) Fc Chimera Protein, Human is more than 95% determined by RP-HPLC.

ELISA

Immobilized CD5(159-372) Fc Chimera Protein, Human (Cat. No. UA016083) at 1.0μg/mL (100μL/well) can bind CD5 Recombinant Rabbit mAb (SDT-2857-16) (Cat. No. S0B2441) with EC50 of 2.54-5.96 ng/mL.