WB result of CD5 Recombinant Rabbit mAb
Primary antibody: CD5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: Raji whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Negative control: Ramos whole cell lysate; Raji whole cell lysate; HeLa whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 58 kDa
This blot was developed with high sensitivity substrate
CD5 Recombinant Rabbit mAb (SDT-2570-53)
CD5 Recombinant Rabbit mAb (SDT-2570-53)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD5 |
| Synonyms | T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; LEU1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P06127 |
| Clone Number | SDT-2570-53 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Jurkat |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
CD5 is a 67 kDa type I cell surface glycoprotein that belongs to the scavenger receptor cysteine-rich (SRCR) family. It is expressed on all T cells and a subset of B cells known as B-1a cells. CD5 plays a crucial role in regulating T cell receptor (TCR) signaling and acts as a negative regulator of T cell activation. It helps fine-tune the threshold for T cell activation during thymocyte development and suppresses peripheral T cell activation by inhibiting TCR-proximal signaling. In B-1a cells, CD5 helps prevent self-reactivity by dampening B cell receptor (BCR) signals. The expression of CD5 is also associated with cell survival, apoptosis, and the development of regulatory T cells. Additionally, CD5 may be involved in the regulation of dendritic cell function and cytokine production.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human skeletal muscle. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD5 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
