CD45 Nanobeads, mouse（RUO）

Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD45 monoclonal antibody

2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations

1 mL magnetic beads: suitable for 5×10⁸total cells, and can perform up to 50 separations

CD45⁺ cells are magnetically labeled using CD45 Nanobeads for mice. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD45⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD45⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive fraction containing CD45⁺ cells must be further separated through a second separation column.

Store protected from light at 2–8°C; do not freeze.