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CD45 Nanobeads, human(RUO)

CD45 Nanobeads, human(RUO)

Catalog Number: S0K0001 Reactivity: Human Conjugation: Brand: Starter
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Product Details

Product Specification


Format

Dextran-coated iron tetroxide nanomagnetic beads coupled with an anti-human CD45 monoclonal antibody

Capacity

2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations

1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 separations

Principle of Separation

CD45⁺ cells are magnetically labeled using CD45 Nanobeads for human samples. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD45⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD45⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD45⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD45⁺ cells must be subjected to a second separation column.

Reactivity Human
Magnetic Bead Size 20 nm
Separation Method Column-Based
Selection Strategy Positive Selection
Stability & Storage

Store protected from light at 2–8°C; do not freeze.

Background

CD45 Nanobeads are designed to positively select or deplete leukocytes from peripheral blood, red blood cell or platelet preparations, lymphoid tissues, tumor tissues, or non-hematopoietic tissues. The CD45 antigen is expressed on all cells of hematopoietic origin, except for red blood cells, platelets, and their precursor cells.

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Validation Data

CD45+ T cells were enriched from or depleted of mixed cultures of C57BL/6 mouse splenocytes and Jurkat cells (human T-cell leukemia T lymphocytes). Subsequently, …Fluorescent staining was performed using anti-CD45 (clone: 30-F11) conjugated to Alexa Fluor™ 488, and the live cell population was gated for analysis.