1μg (R: reducing condition, N: non-reducing condition).
CD45 His Tag Protein, Human
CD45 His Tag Protein, Human
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$560 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | CD45 |
| Synonyms | PTPRC, L-CA, T200 |
| Accession | P08575 |
| Amino Acid Sequence |
Gln26-Lys577,with C-terminal 10*His QSPTPSPTGLTTAKMPSVPLSSDPLPTHTTAFSPASTFERENDFSETTTSLSPDNTSTQVSPDSLDNASAFNTTGVSSVQTPHLPTHADSQTPSAGTDTQTFSGSAANAKLNPTPGSNAISDVPGERSTASTFPTDPVSPLTTTLSLAHHSSAALPARTSNTTITANTSDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFGSPGEPQIIFCRSEAAHQGVITWNPPQRSFHNFTLCYIKETEKDCLNLDKNLIKYDLQNLKPYTKYVLSLHAYIIAKVQRNGSAAMCHFTTKSAPPSQVWNMTVSMTSDNSMHVKCRPPRDRNGPHERYHLEVEAGNTLVRNESHKNCDFRVKDLQYSTDYTFKAYFHNGDYPGEPFILHHSTSYNSKGGGSGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 95-150kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.CD45 in human physiology and clinical medicine. Immunol Lett. 2018 Apr;196:22-32. Epub 2018 Jan 31. |
Background
CD45 is anevolutionary highly conserved receptor protein tyrosine phosphatase exclusivelyexpressed on all nucleated cells of the hematopoietic system. It is characterizedby the expression of several isoforms, specific to a certain cell type and thedevelopmental or activation status of the cell. CD45 is one of the key playersin the initiation of T cell receptor signaling by controlling the activation ofthe Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency results inT- and B-lymphocyte dysfunction in the form of severe combined immunedeficiency. It also plays a significant role in autoimmune diseases and canceras well as in infectious diseases including fungal infections. The knowledgecollected on CD45 biology is rather vast, but it remains unclear whether allfindings in rodent immune cells also apply to human CD45.
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SDS-PAGE
ELISA
Immobilized CD45 His Tag Protein, Human (Cat. No. UA010556) at 2.0μg/mL (100μL/well) can bind CD45 Mouse mAb (S-839-3) (Cat. No. S0B0427) with EC50 of 2.38-2.62ng/mL.
SPR
Anti-His antibody Immobilized on CM5 Chip captured CD45 His Tag Protein, Human (Cat. No. UA010556), can bind Galectin-1 Protein, Human (Cat. No. UA010167) with an affinity constant of 4.78μM as determined in SPR assay.
