2μg (R: reducing condition, N:non-reducing condition).
CD40 Ligand /TNFSF5 Trimer His Tag Protein, Human/Cynomolgus/Rhesus macaque
CD40 Ligand /TNFSF5 Trimer His Tag Protein, Human/Cynomolgus/Rhesus macaque
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$235 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD40 ligand, CD40-L, CD154, TNFSF5 |
| Accession | Human: P29965、 Cynomolgus: G7Q1T4、 Rhesus macaque: P63304 |
| Amino Acid Sequence | It contains Gly116-Leu261 trimer design |
| Expression System | HEK293 |
| Molecular Weight | 50-70kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE,＞90% by HPLC |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1. Michel NA, Zirlik A, Wolf D. CD40L and Its Receptors in Atherothrombosis-An Update. Front Cardiovasc Med. 2017 Jun 20;4:40. |
Background
CD40L (also known as CD154 or gp39, encoded by TNFSF5) is a member of the tumor necrosis factor superfamily expressed as a homotrimeric transmembrane glycoprotein on activated T cells, platelets, and vascular endothelial cells, with an extracellular TNF homology domain that can be cleaved by metalloproteinases to release soluble sCD40L. Through binding to its receptor CD40, CD40L serves as a central regulator of adaptive immunity by facilitating T-B cell crosstalk, driving antibody class switching and germinal center formation, activating dendritic cell maturation, and enhancing cytotoxic T lymphocyte responses. Clinically, sCD40L serves as an independent biomarker for atherosclerosis, acute coronary syndromes, and thrombotic events; CD40L deficiency causes X-linked hyper-IgM syndrome and other immunodeficiencies, whereas its aberrant activation is implicated in autoimmune diseases, transplant rejection, and tumor immune evasion, making the CD40/CD40L axis a critical therapeutic target for cardiovascular and immune-related disorders.
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SDS-PAGE
SEC-HPLC
The purity of CD40 Ligand /TNFSF5 Trimer His Tag Protein, Human/Cynomolgus/Rhesus macaque is more than 90% determined by SEC-HPLC.
ELISA
Immobilized CD40 Fc Chimera Protein, Human (Cat. No. UA010194) at 5.0μg/mL (100μL/well) can bind CD40 Ligand /TNFSF5 Trimer His Tag Protein, Human/Cynomolgus/Rhesus macaque (Cat. No.UA016109) with EC50 of 26.81-40.33ng/mL.
SPR
CD40 Fc Chimera Protein, Human (Cat. No. UA010194) captured on Protein A Chip can bind CD40 Ligand /TNFSF5 Trimer His Tag Protein, Human/Cynomolgus/Rhesus macaque (Cat. No.UA016109) with an affinity constant of 2.48nM as determined in a SPR assay.
