1μg (R: reducing condition, N: non-reducing condition).
CD40 Fc&His Tag Protein, Human
CD40 Fc&His Tag Protein, Human
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$198 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Bp50，CD40 Antigens，CD40，B Cell Surface Antigen CD40，B-Cell Surface Antigen CD40，CDW40，CD40L Receptor，CD40 Molecule，Tumor Necrosis Factor Receptor Superfamily， Member 5，CD40 Molecule， TNF Receptor Superfamily Member 5，TNFRSF5，Tumor Necrosis Factor Receptor Superfamily Member 5，P50，CD40 Antigen，B Cell-Associated Molecule |
| Accession | P25942 |
| Amino Acid Sequence | Glu21-Arg193 with Fc&His Tag at C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 50-60kDa (Reducing) |
| Purity | > 95 % as determined by SDS-PAGE & SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | 1.Hanlon MM, Smith CM, Canavan M, Neto NGB, Song Q, Lewis MJ, O'Rourke AM, Tynan O, Barker BE, Gallagher P, Mullan R, Hurson C, Moran B, Monaghan MG, Pitzalis C, Fletcher JM, Nagpal S, Veale DJ, Fearon U. Loss of synovial tissue macrophage homeostasis precedes rheumatoid arthritis clinical onset. Sci Adv. 2024 Sep 27;10(39):eadj1252. |
Background
CD40, a member of the TNF receptor superfamily, is a glycoprotein crucial for immune responses. It's key for T cell-dependent processes like immunoglobulin class switching, memory B cell development, and germinal center formation. Expressed on B cells, dendritic cells, macrophages, and some tumor cells, CD40 interacts with CD40L to initiate signaling pathways. This interaction is vital for humoral immunity. CD40 defects can lead to hyper-IgM immunodeficiency type 3 (HIGM3), causing issues in B cell isotype switching and antibody responses. CD40's extracellular domain has cysteine-rich repeats, typical of TNF receptors. Early studies emphasized its role in humoral immunity.
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SDS-PAGE
SEC-HPLC
The purity of CD40 Fc&His Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized Biotinylated Human CD40L/CD154/TRAP/gp39 Protein, His&Avi tag (Cat. No. S0A1141) at 2μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind CD40 Fc&His Tag, Human (Cat. No. UA011202) with EC50 of 8.97-16.18ng/ml.
Immobilized Human CD40 Ligand/TNFSF5 protein, His tag (Cat. No. S0A1123) at 5.0μg/mL (100μL/well) can bind CD40 Fc&His Tag, Human (Cat. No. UA011202) with EC50 of 0.12-0.19ug/mL.
