WB result of CD4 Recombinant Rabbit mAb
Primary antibody: CD4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 51-65 kDa
CD4 Recombinant Rabbit mAb (S-6203)
CD4 Recombinant Rabbit mAb (S-6203)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD4 |
| Location | Cell membrane |
| Accession | P06332 |
| Clone Number | S-6203 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Ms |
| Positive Sample | mouse spleen, mouse thymus |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:500 | Ms |
| ICC | 1:50 | Ms |
| IF | 1:500 | Ms |
Background
CD4 is a glycoprotein that functions as a co-receptor for the T-cell receptor (TCR) and is primarily expressed on the surface of helper T cells, monocytes, macrophages, and dendritic cells. It is a member of the immunoglobulin superfamily, consisting of four immunoglobulin domains (D1 to D4) that interact with MHC class II molecules on antigen-presenting cells. This interaction, mediated by the D1 domain of CD4, ensures that T cells recognize antigens presented by MHC II. The cytoplasmic tail of CD4 contains motifs that recruit the tyrosine kinase Lck, which amplifies T cell activation signals. Additionally, CD4 is crucial in the immune response, as it helps activate CD4+ T helper cells, which in turn stimulate other immune cells to combat infections. However, CD4 is also the primary receptor for HIV, allowing the virus to enter host T cells and cause immunodeficiency.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows negative staining in NIH/3T3 cells. Anti-CD4 antibody was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows negative staining in NIH/3T3 cells. Anti-CD4 antibody was used at 1/50 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse thymus. Anti-CD4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
