IF shows positive staining in paraffin-embedded human tonsil. Anti-CD4 (Alexa Fluor® 647 Conjugate) antibody was used at 1/200 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD4 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-R163)
CD4 Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (SDT-R163)
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Product Details
Product Details
Product Specification
| Synonyms | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4 |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | SDT-R163 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| IF | 1:100-1:200 | Hu |
Background
CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It is a type of white blood cell that helps fight infection by triggering your immune system to destroy viruses, bacteria, and other germs that may make you sick. CD4 is also a receptor for the HIV virus, and when the virus infects cells with CD4 surface proteins, it depletes the number of T cells, B cells, natural killer cells, and monocytes in the patient's blood.
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Picture
Immunofluorescence
