IF shows positive staining in paraffin-embedded human tonsil. Anti-CD4 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Orange) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD4 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-R163)
CD4 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-R163)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD4 |
| Synonyms | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4 |
| Location | Cell membrane |
| Accession | P01730 |
| Clone Number | SDT-R163 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | Hu |
Picture
Picture
Immunofluorescence
