CD4+ T cells were enriched from or depleted of spleen cells isolated from BALB/c mice. Subsequently, fluorescent staining was performed using anti-CD4 (clone: GK1.5) Brilliant Violet 421™, and the live cell population was gated for analysis.
CD4 Nanobeads, mouse（RUO）
CD4 Nanobeads, mouse（RUO）
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide nanomagnetic beads conjugated with an anti-mouse CD4 monoclonal antibody |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 separations 1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 separations |
| Principle of Separation | CD4⁺ cells are magnetically labeled using CD4 Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD4⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD4⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD4⁺ cells can be eluted, yielding the positive sorted fraction. To further enhance purity, the positive fraction containing CD4⁺ cells must be subjected to a second separation column. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD4 antigen is expressed on most thymocytes and mature helper T cells, certain NKT cell subsets, and a small fraction of dendritic cell subsets. In CD4+helper T cells, CD4 acts as a co-molecule to assist the TCR heterodimer in recognizing MHC class II/peptide complexes. CD4 antigen is present on approximately 90% of thymocytes, 25% of splenic cells, and 55% of lymph node cells.
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Validation Data
