1 1

CD3E&CD3D Heterodimer, Llama Fc & Llama Fc Tag Protein, Cynomolgus

CD3E&CD3D Heterodimer, Llama Fc & Llama Fc Tag Protein, Cynomolgus

Catalog Number: UA016167 Brand: UA BIOSCIENCE
Share:
Price:
$200 USD
$200 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Human
Synonyms CD3 epsilon&CD3 delta, CD3E & CD3D
Accession Q95LI5-1(CD3E)、Q95LI8-1 (CD3D)
Amino Acid Sequence

Gln 22 - Asp 117 (CD3E) with Llama Fc Tag at the C-Terminus;
Phe 22 - Ala 105 (CD3D) with Llama Fc Tag at the C-Terminus
Expression System HEK293
Molecular Weight 40-55kDa (Reducing)
Purity ＞95% by SDS-PAGE & HPLC
Conjugation Unconjugated
Physical Appearance Lyophilized Powder
Storage Buffer

PBS, pH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. Wang, Z., et al. (2004). Expression and characterization of recombinant soluble monkey CD3 molecules: mapping the FN18 polymorphic epitope. Molecular Immunology, 40(16), 1179-1188.
2. Uda, A., et al. (2003). Identification of an amino acid responsible for the CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis). Journal of Medical Primatology, 32(2), 105-110.
3. Uda, A., et al. (2004). CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis). Journal of Medical Primatology, 33(1), 34-40.

Background

Cynomolgus macaque (Macaca fascicularis) CD3E (CD3 epsilon chain) and CD3D (CD3 delta chain) are two key subunits of the T cell receptor (TCR)-CD3 complex, both of which are single-pass type I membrane glycoproteins. These two subunits form a CD3εδ heterodimer on the cell surface, serving as one of the core signal-transducing components of the TCR-CD3 complex.

The ectodomains of cynomolgus CD3E and CD3D correspond to Gln22-Asp117 of CD3E and Phe22-Ala105 of CD3D, respectively. The UniProt accession numbers are Q95LI5 for CD3E and Q95LI8 for CD3D. Due to glycosylation, recombinant CD3E&CD3D heterodimers typically exhibit higher apparent molecular weights on SDS-PAGE than their calculated values.

CD3E and CD3D, together with CD3G and CD3Z (CD247), constitute the signaling module of the TCR-CD3 complex. When antigen-presenting cells (APCs) activate the T cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains (CD3D, CD3E, CD3G, and CD3Z). The immunoreceptor tyrosine-based activation motifs (ITAMs) within the cytoplasmic tails of CD3 subunits become phosphorylated, creating docking sites for the protein kinase ZAP-70 and initiating downstream signaling cascades

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

RP-HPLC

The purity of CD3E&CD3D Heterodimer, Llama Fc & Llama Fc Tag Protein, Cynomolgus is more than 95% determined by RP-HPLC.

ELISA

Immobilized CD3E&CD3D Heterodimer, Llama Fc & Llama Fc Tag Protein, Cynomolgus (UA016167) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) with a linear range of 10.68-13.82 ng/mL.