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CD3ε Nanobeads Kit, mouse (RUO)

CD3ε Nanobeads Kit, mouse (RUO)

Catalog Number: S0K0015 Reactivity: Mouse Conjugation: Brand: Starter
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$335 USD
$335 USD
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Product Details

Product Specification


Format

Biotinylated anti-mouse CD3ε monoclonal antibody and streptavidin-conjugated nanomagnetic beads, in a PBS buffer system containing BSA and Poloxamer 188.
Capacity

1 mL magnetic beads: Suitable for a total cell count of 5×10⁸, allowing for up to 50 sorting runs.

2 mL magnetic beads: Suitable for a total cell count of 1×10⁹, allowing for up to 100 sorting runs.
Principle of Separation

First, CD3ε+ cells are labeled with a CD3ε-biotin–conjugated primary antibody. Subsequently, the cells are magnetically labeled using biotin-conjugated nanomagnetic beads. The cell suspension is then placed in a separation column within a magnetic field. The CD3ε+ cells that have been labeled with the magnetic beads remain retained in the column, while the unlabeled cells flow through; thus, CD3ε+ cells are separated from the other cell populations.
Reactivity Mouse
Magnetic Bead Size 20 nm
Separation Method Column-Based
Selection Strategy Positive Selection
Stability & Storage

Store protected from light at 2–8 °C; do not freeze.

Background

CD3ε is a transmembrane protein with a molecular weight of 20 kDa and serves as a subunit of the T-cell receptor (TCR) complex. Together with the γ and δ subunits of CD3, it plays a crucial role in the assembly and expression of the TCR complex. CD3 is expressed on the surface of thymocytes, mature T lymphocytes, and natural killer T cells (NKT cells). Using the CD3ε nanosphere kit, mouse T lymphocytes and NKT cells can be depleted from various tissues, including the spleen, thymus, and lymph nodes.

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Validation Data

Using the CD3ε Nanobeads Kit, mouse S(D) separation columns, and a sorter, CD3ε-positive cells were isolated from the single-cell suspension. The cells were fluorescently labeled with an anti-CD3ε+ (clone: S-R491) Alexa Fluor® 488 antibody (SDT Cat: S0B5473), and the "live cell+" population was selected.