Enrich or deplete T cells from human PBMCs. Cells were stained with anti-CD3 (clone: UCHT1) Brilliant Violet 421™, and the live cell population was gated for analysis.
CD3 Nanobeads, human（RUO）
CD3 Nanobeads, human（RUO）
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD3 monoclonal antibody. |
| Capacity |
2 mL magnetic beads: suitable for a total cell count of 1×10⁹, and can perform up to 100 sorting runs 1 mL magnetic beads: suitable for 5×108total cells, and can perform up to 50 sorting runs |
| Principle of Separation | CD3 Nanobeads are used to magnetically label human CD3⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD3⁺ cells remain retained in the column, while the unlabeled cells flow through; this fraction constitutes the negative fraction after removal of CD3⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD3⁺ cells can be eluted, yielding the positive sorted fraction. If higher purity is required, the positive sorted fraction containing CD3⁺ cells must be further separated using a second column. |
| Reactivity | Human |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD3 is expressed on the surface of all T cells and is associated with the T-cell receptor. In human peripheral blood lymphocytes, 70–80% are CD3+, and in thymocytes, 65–85% are CD3+. The antigenic epitope recognized by CD3 Nanobeads is located on the CD3ε chain.
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Validation Data
