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CD3 gamma His Tag Protein, Human

CD3 gamma His Tag Protein, Human

Catalog Number: UA016025 Brand: UA BIOSCIENCE
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$230 USD
$230 USD
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Product Details

Product Specification


Species Human
Synonyms CD3 gamma, CD3G
Accession P09693
Amino Acid Sequence

Gln 23 - Ser 116 with His Tag at the C-Terminus
Expression System HEK293
Molecular Weight

15-25kDa (Reducing)

Purity ＞95% by SDS-PAGE & HPLC
Conjugation Unconjugated
Tag His Tag
Physical Appearance Lyophilized powder
Storage Buffer

PBS, PH7.4, 5% trehalose
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
· 3 months, -20 to -80℃ under sterile conditions after reconstitution.
· 1 week, 2 to 8℃ under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.
Reference

1. The role of the different CD3γ domains in TCR expression and signaling. Frontiers in Immunology, 13, 978658.
2. Signal transduction by lymphocyte antigen receptors. Cell, 76(2), 263–274.
3. Deconstructing the form and function of the TCR/CD3 complex. Immunity, 24(2), 133–139.

Background

CD3γ (Cluster of Differentiation 3 gamma) is a critical subunit of the T cell receptor (TCR) complex. It is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. The CD3γ chain, together with CD3δ, CD3ε, and the CD3ζ homodimer, forms the invariant CD3 signaling module that associates with the variable TCRαβ or TCRγδ chains.

The primary function of CD3γ is to mediate signal transduction upon antigen recognition. Like other CD3 subunits, CD3γ contains three immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain. Upon TCR engagement with peptide-MHC complexes, these ITAMs are phosphorylated by Src-family kinases (LCK and FYN), initiating downstream signaling cascades that lead to T cell activation, proliferation, and differentiation.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of CD3 gamma His Tag Protein, Human is more than 95% determined by SEC-HPLC.

RP-HPLC

The purity of CD3 gamma His Tag Protein, Human is more than 95% determined by RP-HPLC.