1μg (R: reducing conditions, N: non-reducing conditions).
CD3 gamma Fc Chimera Protein, Human
CD3 gamma Fc Chimera Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | CD3 gamma |
| Synonyms | T-cell receptor T3 gamma chain, T-cell surface glycoprotein CD3 gamma chain |
| Accession | P09693 |
| Amino Acid Sequence | Gln23-Ser116, with C-terminal Human IgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 43-50kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Michael S Kuhns, Mark M Davis, K Christopher Garcia. Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9. |
Background
T-cell surface glycoprotein CD3 gamma chain, also known as CD3G, is a 20 kDa single-pass type I membrane protein that is part of the TCR-CD3 complex. CD3 gamma is located on human chromosome 11 and mouse chromosome 9. CD3 gamma, a subunit of the T cell receptor-CD3 (TCR/CD3) complex, helps to support surface TCR/CD3 expression and participates in signal transduction for gene induction after antigen recognition by T lymphocytes, and in TCR/CD3 down-modulation. The TCR/CD3 complex is assembled after a series of pairwise interactions involving the formation of dimers of CD3 epsilon with either CD3 gamma or CD3 delta. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z.
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SDS-PAGE
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 gamma Fc Chimera (Cat. No.UA010598) is immobilized at 1.0 μg/mL, the concentration of Biotinylated Recombinant Human CD3E Fc Chimera(Cat. No. UA010199) that produces 50% of the optimal binding response is approximately 2.2-3.2 μg/mL.
