ICC shows positive staining in Jurkat cells. Anti-CD3 epsilon (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
CD3 epsilon Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-241-49)
CD3 epsilon Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (S-241-49)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD3 EPSILON |
| Synonyms | T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, T3E, CD3E |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P07766 |
| Clone Number | S-241-49 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | null |
| ICC | 1:200 | null |
Background
CD3 (cluster of differentiation 3) is a protein complex and T cell co-receptor that is involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the CD3-zeta (ζ-chain) to generate an activation signal in T lymphocytes. The TCR, CD3-zeta, and the other CD3 molecules together constitute the TCR complex. The CD3–T cell receptor (TCR) complex plays a central role in the T-cell-mediated immunoresponse as it is involved in the recognition of antigens and subsequent signal transduction and activation of immunocompetent T lymphocytes. Because CD3 is required for T cell activation, drugs (often monoclonal antibodies) that target it are being investigated as immunosuppressant therapies (e.g., otelixizumab, teplizumab) for type 1 diabetes and other autoimmune diseases.
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Immunocytochemistry
Negative control：ICC shows negative staining in Ramos cells. Anti-CD3 epsilon (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Immunofluorescence
IF shows positive staining in paraffin-embedded human spleen. Anti-CD3 epsilon (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human tonsil. Anti-CD3 epsilon (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
