2μg (R: reducing condition, N: non-reducing condition).
CD3 epsilon Fc&His Tag Protein, Human
CD3 epsilon Fc&His Tag Protein, Human
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$235 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD3 epsilon, CD3E |
| Accession | P07766-1 |
| Amino Acid Sequence | Asp 23 - Asp 126 (CD3E) with hIgG1 Fc Tag at the C-Terminus, followed by polyhistidine tag |
| Expression System | HEK293 |
| Molecular Weight | 40-55kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & HPLC |
| Conjugation | Unconjugated |
| Tag | Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Severe combined immunodeficiency caused by deficiency in either the CD3 epsilon or the CD3 gamma subunit of the T-cell antigen receptor complex. Journal of Clinical Investigation, 114(10), 1512-1517. |
Background
The CD3E (CD3 epsilon subunit of T-cell receptor complex) gene encodes the CD3ε chain of the T-cell surface glycoprotein, an essential component of the T cell receptor (TCR)-CD3 complex. The gene is located on human chromosome 11q23.3, and the CD3ε protein is a single-pass type I transmembrane protein belonging to the immunoglobulin (Ig) superfamily.
Structure and Function: Human CD3ε protein consists of an extracellular domain (104 amino acids), a transmembrane domain (26 amino acids), and a cytoplasmic domain (55 amino acids). The extracellular region contains an immunoglobulin-like domain, and the cytoplasmic tail contains an immunoreceptor tyrosine-based activation motif (ITAM) domain. CD3ε, together with CD3γ, CD3δ, and CD3ζ, forms the signaling module of the TCR-CD3 complex. Upon TCR engagement by antigen-presenting cells, the ITAM motifs of CD3 chains become phosphorylated, initiating downstream signaling cascades that convert antigen recognition into intracellular activation signals.
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SDS-PAGE
SEC-HPLC
The purity of CD3 epsilon Fc&His Tag Protein, Human is more than 95% determined by SEC-HPLC.
ELISA
Immobilized Anti Human CD3 MAb, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind CD3 epsilon Fc&His Tag Protein, Human (Cat. No. UA016209) with a linear range of 1.2-1.7 ng/mL.
