IF shows positive staining in paraffin-embedded human tonsil. Anti-CD23 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD23 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-028-67)
CD23 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-028-67)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD23 |
| Synonyms | BLAST-2, C-type lectin domain family 4 member J,Fc-epsilon-RII,Immunoglobulin E-binding factor, Lymphocyte IgE receptor, FCER2; CD23A,CLEC4J,FCE2, IGEBF |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P06734 |
| Clone Number | SDT-028-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:50 | Hu |
Background
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. CD23 is known to have a role of transportation in antibody feedback regulation. Antigens which enter the blood stream can be captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell lymphoma (CD23-negative). CD23 can also be demonstrated in germinal centre follicular dendritic cells using immunohistochemistry but is minimally expressed by benign germinal center B cells.
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Immunofluorescence
Negative control: IF shows negative staining in paraffin-embedded human kidney. Anti-CD23 (Alexa Fluor® 700 Conjugate) antibody was used at 1/50 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
