2μg (R: reducing condition, N: non-reducing condition).
CD205 His Tag Protein, Cynomolgus
CD205 His Tag Protein, Cynomolgus
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$215 USD
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | Ly-75,DEC-205,CD205,CLEC13B,GP200-MR6,LY75,DEC205 |
| Accession | XP_005573337.2 |
| Amino Acid Sequence | Ser28-Asp1666 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | ＞180kDa(Reducing) |
| Endotoxin | <1EU/μg |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation |
| Stability & Storage |
· 12 months from date of receipt,lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃under sterile conditions after reconstitution. · 1 week, 2 to 8℃under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Bhardwaj N, Friedlander PA, Pavlick AC, Ernstoff MS, Gastman BR, Hanks BA, Curti BD, Albertini MR, Luke JJ, Blazquez AB, Balan S, Bedognetti D, Beechem JM, Crocker AS, D'Amico L, Danaher P, Davis TA, Hawthorne T, Hess BW, Keler T, Lundgren L, Morishima C, Ramchurren N, Rinchai D, Salazar AM, Salim BA, Sharon E, Vitale LA, Wang E, Warren S, Yellin MJ, Disis ML, Cheever MA, Fling SP. Flt3 ligand augments immune responses to anti-DEC-205-NY-ESO-1 vaccine through expansion of dendritic cell subsets. Nat Cancer. 2020 Dec;1(12):1204-1217. 2.Lahoud MH, Ahmet F, Zhang JG, Meuter S, Policheni AN, Kitsoulis S, Lee CN, O'Keeffe M, Sullivan LC, Brooks AG, Berry R, Rossjohn J, Mintern JD, Vega-Ramos J, Villadangos JA, Nicola NA, Nussenzweig MC, Stacey KJ, Shortman K, Heath WR, Caminschi I. DEC-205 is a cell surface receptor for CpG oligonucleotides. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16270-5. |
Background
CD205 (also known as DEC-205/LY75) is a type I transmembrane glycoprotein belonging to the macrophage mannose receptor family. Structurally, it comprises an N-terminal cysteine-rich domain, a fibronectin type II domain, and ten C-type lectin-like domains; cryo-electron microscopy has revealed that its monomer forms two mutually interdigitated lectin rings and exhibits pH-dependent conformational changes associated with oligomerization. Functionally, CD205 serves as a prototypical endocytic receptor on dendritic cells, rapidly internalizing antigens and directing them to specialized antigen-processing compartments, thereby participating in cross-presentation and the recognition of apoptotic/necrotic cells. Clinically, CD205 is highly expressed in various solid tumors (such as triple-negative breast cancer and pancreatic cancer) and lymphomas, making it an active target for antibody-drug conjugates (e.g., MEN1309/OBT076); its antigen-delivery capacity has also been exploited in the development of tolerogenic vaccine strategies.
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SDS-PAGE
SEC-HPLC
The purity of CD205 His Tag Protein, Cynomolgus is more than 90% determined by SEC-HPLC.
ELISA
Immobilized CD205 His Tag Protein, Cynomolgus (Cat. No.UA016201) at 2.0μg/mL (100μL/well) can bind Oberotatug with EC50 of 0.087-0.5ng/mL.
