CD1c Recombinant Rabbit mAb (S-5242)

Catalog Number: S0B60027 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	CD1c
Synonyms	T-cell surface glycoprotein CD1c; CD1C
Location	Cell membrane, Endosome, Lysosome
Accession	P29017
Clone Number	S-5242
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Jurkat, Molt-4
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500	Hu

Background

CD1c is a lipid-presenting, MHC class I-like glycoprotein expressed on dendritic cells and B cells that binds both self and foreign lipids/glycolipids within its deep hydrophobic groove and shuttles them through endosomal compartments to display them to αβ and γδ T cells, thereby bridging innate and adaptive immunity; uniquely, its open F′ pocket can accommodate both lipid tails and peptide moieties of lipopeptides, enabling recognition of mycobacterial antigens such as mannosyl-β1-phosphomycoketide and driving potent IFN-γ responses essential for controlling intracellular pathogens like Mycobacterium tuberculosis.

Picture

Western Blot

WB result of CD1c Rabbit Recombinant Rabbit mAb
Primary antibody: CD1c Rabbit Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: THP-1 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Negative control: THP-1 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 39 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thymus. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD1c antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.