Use the CD19 Nanobeads Kit, mouse S or L Separation Column, and a magnetic sorter to enrich or deplete CD19+ cells from mouse spleens. Subsequently, perform fluorescent staining with Alexa Fluor® 647 anti-mouse CD19 Antibody (6D5) and Brilliant Violet 421™ anti-mouse CD3 Antibody (Clone: 17A2), and gate on the live cell population for analysis.
CD19 Nanobeads, mouse（RUO）
CD19 Nanobeads, mouse（RUO）
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$210 USD
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-mouse CD19 monoclonal antibody |
| Capacity |
1 mL magnetic beads: suitable for a total cell count of 5×108and can perform up to 50 sorting runs. 2 mL magnetic beads: suitable for a total cell count of 1×109and can perform up to 100 sorting runs. |
| Principle of Separation | CD19 Nanobeads, mouse are used to magnetically label CD19⁺ cells. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD19⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD19⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD19⁺ cells can be eluted, yielding the positive sorted fraction. |
| Applications in cell sorting | Isolate or deplete CD19+ cells from a single-cell suspension of mouse spleen. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
Mouse CD19 is encoded by the Cd19 gene and is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, serving as a classic marker of the B-cell lineage. This molecule begins to be expressed at the pro-B-cell stage in the bone marrow and remains on the surface of the vast majority of mature B cells, while its expression is downregulated in terminally differentiated plasma cells.
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Validation Data
DLS
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