WB result of CD177 Recombinant Rabbit mAb
Primary antibody: CD177 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated 293F whole cell lysate 20 μg
Lane 2: 293F transfected with CD177-myc-His fusion protein whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 16-65 kDa
CD177 Recombinant Rabbit mAb (S-4425-31)
CD177 Recombinant Rabbit mAb (S-4425-31)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD177 |
| Immunogen | Synthetic Peptide |
| Location | Secreted, Cell membrane |
| Accession | Q8N6Q3 |
| Clone Number | S-4425-31 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:2000 | Hu |
| IF | 1:2000 | Hu |
Background
CD177 (also known as NB1 glycoprotein or HNA-2a antigen) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein of approximately 50–60 kDa belonging to the Ly-6/uPAR superfamily, expressed mainly on the plasma membrane and secondary granules of neutrophils, and present only on a subset of about 45%–65% of neutrophils, with the expressing proportion varying among healthy individuals. Its classic function is to serve as a high-affinity receptor for proteinase 3 (PR3) on the neutrophil surface, forming the CD177–PR3 membrane complex that maintains high-density presentation of PR3 on the cell surface; this complex constitutes a key target for autoantibody attack in PR3-ANCA-associated vasculitis, and blocking the binding of CD177 to PR3 can significantly reduce ANCA-induced neutrophil respiratory burst. In addition, CD177 can participate in neutrophil transendothelial migration by binding PECAM-1 on endothelial cells, and may influence the balance between adhesion and migration by regulating β2 integrin signaling. Recent studies have further revealed that CD177 is also expressed on the surface of some tumor-infiltrating regulatory T cells and is associated with enhanced immunosuppressive activity and tumor immune evasion. Clinically, absence of CD177 expression is associated with the HNA-2-negative phenotype and can lead to transfusion-related acute lung injury and neonatal alloimmune neutropenia, while changes in the proportion of CD177-expressing neutrophils are also regarded as a potential biomarker for diseases such as myeloproliferative neoplasms and inflammatory bowel disease.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-CD177 antibody was used at 1/2000dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-CD177 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-CD177 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD177 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD177 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human colon. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human colon cancer. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human lung squamous carcinoma. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD177 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
