WB result of CD151 Recombinant Rabbit mAb
Primary antibody: CD151 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled Daudi whole cell lysate 20 µg
Lane 2: unboiled Molt-4 whole cell lysate 20 µg
Lane 3: unboiled A431 whole cell lysate 20 µg
Lane 4: unboiled HepG2 whole cell lysate 20 µg
Lane 5: unboiled A549 whole cell lysate 20 µg
Lane 6: unboiled A172 whole cell lysate 20 µg
Lane 7: unboiled MDA-MB-231 whole cell lysate 20 µg
Negative control: unboiled Daudi whole cell lysate; unboiled Molt-4 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 25 kDa
CD151 Recombinant Rabbit mAb (S-3235-104)
CD151 Recombinant Rabbit mAb (S-3235-104)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD151 |
| Synonyms | CD151 antigen; GP27; Membrane glycoprotein SFA-1; Platelet-endothelial tetraspan antigen 3 (PETA-3); Tetraspanin-24 (Tspan-24); TSPAN24 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Synapse |
| Accession | P48509 |
| Clone Number | S-3235-104 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HepG2, A549, A172, MDA-MB-231, bEND.3, mouse kidney, rat kidney |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2500 | Hu |
| IF | 1:250 | Hu |
Background
CD151, also known as tetraspanin-24 or PETA-3, is a widely expressed transmembrane glycoprotein belonging to the tetraspanin superfamily that plays a pivotal role in regulating cell adhesion, migration, and signaling by organizing multimolecular complexes within the plasma membrane. Functioning primarily as a molecular organizer, CD151 forms stable associations with integrins, particularly laminin-binding integrins such as α3β1, α6β1, and α6β4, thereby strengthening cell-matrix interactions and facilitating the assembly of specialized microdomains often referred to as "tetraspanin webs" that recruit various signaling molecules, growth factor receptors, and proteases. This protein is critically involved in diverse physiological processes including embryonic development, angiogenesis, wound healing, and platelet function, while its dysregulation is strongly implicated in pathological conditions, most notably cancer progression, where elevated CD151 expression correlates with enhanced tumor cell motility, invasion, metastasis, and poor prognosis in carcinomas of the breast, lung, liver, and pancreas by modulating integrin-dependent signaling pathways like PI3K/Akt and MAPK/ERK; furthermore, CD151 serves as an entry cofactor for certain viruses, such as hepatitis C virus and human papillomavirus, making it a significant target for therapeutic interventions aimed at inhibiting metastatic spread or viral infection.
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Western Blot
WB result of CD151 Recombinant Rabbit mAb
Primary antibody: CD151 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled EL4.IL2 whole cell lysate 20 µg
Lane 2: unboiled bEnd.3 whole cell lysate 20 µg
Lane 3: unboiled mouse kidney lysate 20 µg
Negative control: unboiled EL4.IL2 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
WB result of CD151 Recombinant Rabbit mAb
Primary antibody: CD151 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat liver lysate 20 µg
Lane 2: unboiled rat kidney lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 28 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-CD151 antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded Human colon cancer. Anti- CD151 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded Human lung adenocarcinoma. Anti- CD151 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded Human ovarian cancer. Anti- CD151 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded Human endometrial cancer. Anti- CD151 antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
