CD15 Nanobeads, human（RUO）

Catalog Number: S0K0025 Reactivity: Human Conjugation: Brand: Starter

Price:

$450 USD

Size: 1 mL 2 mL

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Format	Dextran-coated iron tetroxide magnetic nanoparticles conjugated with an anti-human CD15 monoclonal antibody.
Capacity	1 mL magnetic beads: suitable for a total cell count of 5×10⁸and can perform up to 50 sorting runs. 2 mL magnetic beads: suitable for a total cell count of 1×10⁹and can perform up to 100 sorting runs.
Principle of Separation	CD15⁺ cells are magnetically labeled using CD15 Nanobeads. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD15⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD15⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD15⁺ cells can be eluted, yielding the positive sorted fraction.
Applications in cell sorting	Eosinophils and neutrophils can be isolated from or depleted from tissue samples, such as human peripheral blood or ovarian cancer tissues.
Reactivity	Human
Magnetic Bead Size	20 nm
Separation Method	Column-Based
Selection Strategy	Positive Selection
Stability & Storage	Store protected from light at 2–8°C; do not freeze.

Background

CD15 Nanobeads, human are designed to positively select or deplete CD15-positive cells from human peripheral blood or tissue single-cell suspensions. The CD15 antigen is expressed on neutrophils, eosinophils, and myeloid lineage monoblast progenitor cells, but it is not expressed on basophils or lymphocytes. Furthermore, this antigen is also expressed in Hodgkin/Reed-Sternberg cells.

Protocol

Note: This kit does not include the sorting buffer; it must be provided separately. The corresponding Stater product number is S0D3018.

Taking SSeparation Columnas an example

	Steps	Operating procedures	Dosage / Time
Magnetic labeling	1	Count the total number of cells after erythrocyte lysis of human peripheral blood Note:It is recommended to count cells after staining with AO/PI	—
	2	Centrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant	400×g, 7 min
	3	Resuspend every 1×10⁷ cells in 80 μL of buffer Note:The amount of buffer should be adjusted proportionally according to the sample volume.	Resuspend the cells.
	4	Add 20 μL per 1×10⁷ cells.CD15 Nanobeads, human. Note:When the cell quantity increases, all reagent volumes should be increased proportionally.	Add 20 μL per 1×10⁷ cells.
	5	Mix thoroughly and incubate in the dark at 2–8℃ for 15 minutes.	Incubate for 15 min
	6	Wash every 1×10⁷ cells with 1 mL of buffer, centrifuge at 400×g for 7 minutes, and completely discard the supernatant	Add 1 mL to 1×10⁷ cells
	7	Resuspend up to 1×10 in 500 μL of buffer⁸Cells	Resuspend the cells
Magnetic separation	8	Place the S-type separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 500 μL of buffer	Rinse the separation column
	9	Load the cell suspension onto the separation column, and collect the flow-through (unlabeled cells)	—
	10	Wash the column with buffer three times, 500 μL each time; add the next wash only after the liquid in the column has completely drained	3 × 500 μL of buffer
	11	Combine all flow-through fractions into the negative fraction; remove the separation column from the magnetic field and place it over the collection tube	—
	12	Add 1 mL of buffer to the column, and quickly and firmly push the accompanying plunger to elute the magnetically labeled positive cells. Note:For higher purity, use a new column and repeat the separation once.	Collect the target cells.

With LSeparation Columnas an example.

	Steps	Operating method	Dosage / Time
Magnetic labeling	1	Count the total number of cells after erythrocyte lysis of human peripheral blood Note:It is recommended to count cells after staining with AO/PI.	—
	2	Centrifuge the cell suspension at 400×g for 7 minutes, and completely discard the supernatant.	400×g, 7 min
	3	Resuspend every 1 × 10⁷ cells in 80 μL of buffer. Note:Adjust the volume of the buffer proportionally according to the sample volume.	Resuspend the cells.
	4	Add 20 μL per 1 × 10⁷ cells.CD15 Nanobeads, human. Note:When the cell quantity increases, increase the volume of all reagents proportionally at the same time.	Add 20 μL per 1 × 10⁷ cells.
	5	Mix thoroughly, incubate at 2–8°C in the dark for 15 minutes	Incubate for 15 min
	6	Add 1 mL of buffer to wash every 1×10⁷ cells, centrifuge at 400×g for 7 minutes, and completely discard the supernatant	1×10⁷ cells plus 1 mL
	7	Resuspend up to 1×10 in 500 μL of buffer⁸Cells	Resuspend the cells
Magnetic cell sorting	8	Place the L-shaped separation column into the magnetic field of the corresponding magnetic separator, and rinse the column with 3 mL of buffer	Rinse the separation column
	9	Load the cell suspension onto the separation column, and collect the effluent (unlabeled cells)	—
	10	Wash the column three times with 3 mL of buffer each time, adding the next rinse only after the liquid inside the column has completely drained	3 × 3 mL of buffer
	11	Combine all effluents into the negative fraction; remove the separation column from the magnetic field and place it over the collection tube	—
	12	Add 5 mL of buffer to the column, and quickly and firmly push the accompanying plunger to elute the magnetically labeled positive cells. Note:For higher purity, use a new column and repeat the separation once.	Collect the target cells.

Picture

Validation Data

Use CD15 Nanobeads, human S or L Separation Columns, and a magnetic sorter to enrich or deplete CD15+ cells from human peripheral blood. Subsequently, perform fluorescent staining with APC anti-human CD15 (SSEA-1) Antibody (Clone: HI98), and gate on the live cell population for analysis.

Item added to your cart

CD15 Nanobeads, human（RUO）