CD134 Recombinant Rabbit mAb (S-4797)

Catalog Number: S0B6843 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CD134
Synonyms	Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; Ox40; Txgp1; Tnfrsf4
Location	Membrane
Accession	P47741
Clone Number	S-4797
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Ms, Rt
Positive Sample	mouse splenocytes treated with 2.5 μg/ml concanavalin A for 72 hours, mouse spleen, rat spleen
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms, Rt
IHC-P	1:100	Ms, Rt

Background

CD134, also known as OX40 or TNFRSF4, is a costimulatory molecule belonging to the tumor necrosis factor receptor (TNFR) superfamily that is primarily expressed on the surface of activated CD4+ and CD8+ T cells, playing a pivotal role in regulating adaptive immune responses by binding to its ligand, OX40L (CD252), which is typically found on antigen-presenting cells such as dendritic cells, B cells, and macrophages. This interaction delivers a critical secondary signal that enhances T cell proliferation, survival, and cytokine production, particularly by upregulating anti-apoptotic proteins like Bcl-2 and Bcl-xL, thereby preventing activation-induced cell death and promoting the formation of long-lived memory T cells. Due to its significant influence on sustaining T cell activity, CD134 has emerged as a major target in cancer immunotherapy, where agonistic antibodies are designed to boost anti-tumor immunity, while blocking antibodies are explored for treating autoimmune diseases by dampening excessive immune responses, highlighting its dual potential in both enhancing and suppressing immune pathways depending on the clinical context.

Picture

Western Blot

WB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated mouse splenocytes whole cell lysate 20 µg
Lane 2: mouse splenocytes treated with 2.5 μg/ml concanavalin A for 72 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDa

WB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Negative control: mouse brain lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDa

WB result of CD134 Recombinant Rabbit mAb
Primary antibody: CD134 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 30 kDa
Observed MW: 47 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-CD134 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat spleen. Anti-CD134 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.