CD127 Recombinant Rabbit mAb (S-4826)

Catalog Number: S0B6827 Application: IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CD127
Synonyms	Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RAAlternative nameCDw127; IL7R
Location	Secreted, Cell membrane
Accession	P16871
Clone Number	S-4826
Antibody Type	Recombinant mAb
Isotype	IgG
Application	IHC-P
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
IHC-P	1:250	Hu

Background

CD127, also known as the alpha chain of the interleukin-7 receptor (IL-7Rα), is a type I transmembrane glycoprotein that plays a pivotal role in lymphocyte development, survival, and homeostasis by pairing with the common gamma chain (CD132) to form the functional IL-7 receptor complex. Upon binding to its ligand, IL-7, CD127 triggers downstream signaling cascades, primarily through the JAK1/STAT5 pathway, which are essential for the generation and maintenance of naïve and memory T cells, as well as early B cell development in the bone marrow. Beyond its physiological importance in adaptive immunity, CD127 serves as a critical phenotypic marker in immunology and oncology; it is characteristically expressed on naïve and central memory T cells but downregulated on terminally differentiated effector cells and regulatory T cells (Tregs), making it a valuable tool for distinguishing Treg populations (typically CD4+CD25highCD127low/neg) from activated conventional T cells. Furthermore, aberrant expression or mutations in the gene encoding CD127 (IL7R) have been implicated in various pathologies, including severe combined immunodeficiency (SCID), multiple sclerosis susceptibility, and certain leukemias, highlighting its significance as both a diagnostic marker and a potential therapeutic target for modulating immune responses in autoimmunity, cancer immunotherapy, and immune reconstitution strategies.

Picture

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD127 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.