Using the CD11c Nanobeads Kit, mouse S(L) separation columns, and a cell sorter, CD11c-positive cells were isolated from a single-cell suspension of mouse spleen. Subsequently, fluorescent staining was performed with APC Armenian Hamster Anti-Mouse CD11c Antibody (N418) (STD Cat: S0B5085) and FITC Rat Anti-Mouse I-A/I-E Antibody (M5/114.15.2) (STD Cat: S0B1661), and the live cell population was gated for analysis.
CD11c Nanobeads Kit, mouse (RUO)
CD11c Nanobeads Kit, mouse (RUO)
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Product Details
Product Details
Product Specification
| Format | Dextran-coated iron oxide nanomagnetic beads conjugated with an anti-mouse CD11c monoclonal antibody, in a PBS buffer system containing BSA and Poloxamer 188. |
| Capacity |
1 mL magnetic beads: suitable for 1×10nineTotal cell count: up to 100 sorting runs can be performed. 2 mL magnetic beads: suitable for a total cell count of 2 × 10⁹, enabling up to 200 sorting runs. |
| Principle of Separation | CD11c⁺ cells are magnetically labeled using CD11c Nanobeads, mouse. The cell suspension is then loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD11c⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after depletion of CD11c⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD11c⁺ cells can be eluted, yielding the positive sorted fraction. |
| Applications in cell sorting | Isolate or deplete CD11c+ cells from a single-cell suspension of mouse spleen. |
| Reactivity | Mouse |
| Magnetic Bead Size | 20 nm |
| Separation Method | Column-Based |
| Selection Strategy | Positive Selection |
| Stability & Storage | Store protected from light at 2–8°C; do not freeze. |
Background
CD11c Nanobeads are specifically designed for the isolation of CD11c-positive cells (such as dendritic cells, DCs) from single-cell suspensions derived from murine lymphoid and non-lymphoid tissues. Unlike human cells, all known subsets of dendritic cells in mice express CD11c, and certain tissue-resident macrophage subsets also carry this marker. By using these CD11c magnetic beads, you can replace the traditionally laborious and complex steps of dendritic cell isolation with a simple and rapid positive-selection protocol.
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Validation Data
