CD11b NanoBeads, human and mouse（RUO）

Dextran-coated iron oxide nanomagnetic beads conjugated with anti-human and anti-mouse CD11b monoclonal antibodies, in a PBS buffer system containing BSA and Poloxamer 188.

1. For human whole blood or mouse bone marrow cells: Add 20 μL of nanobeads per 10⁷ cells, allowing for up to 100 separations. 2. For human PBMCs: Add 6 μL of nanobeads per 10⁷ cells, allowing for up to 333 separations. 3. For mouse splenocytes: Add 2 μL of nanobeads per 10⁷ cells, allowing for up to 1,000 separations.

AdoptCD11b NanoBeads, human and mouseMagnetic labeling of CD11b⁺ cells. Subsequently, the cell suspension is loaded into a separation column placed within the sorter's magnetic field. The magnetically labeled CD11b⁺ cells remain retained in the column, while the unlabeled cells elute; this fraction constitutes the negative fraction after removal of CD11b⁺ cells. After removing the separation column from the magnetic field, the magnetically captured CD11b⁺ cells can be eluted, yielding the positive sorted fraction.

Store protected from light at 2–8 °C; do not freeze.