CCDC47 Recombinant Rabbit mAb (S-4158-17)

Catalog Number: S0B60026 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100µl

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Product Details

Product Specification

Host	Rabbit
Antigen	CCDC47
Synonyms	PAT complex subunit CCDC47; Calumin; Coiled-coil domain-containing protein 47
Immunogen	Synthetic Peptide
Location	Endoplasmic reticulum
Accession	Q96A33
Clone Number	S-4158-17
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HeLa, Jurkat, HEK-293
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu
IHC-P	1:500	Hu

Background

CCDC47 (Coiled-Coil Domain Containing Protein 47) is a multifunctional transmembrane protein primarily localized to the endoplasmic reticulum, playing a critical role in cellular calcium homeostasis regulation, protein processing, and the unfolded protein response. This protein is characterized by an N-terminal signal peptide, a central region containing multiple predicted coiled-coil domains that mediate protein-protein interactions, and a C-terminal transmembrane domain that anchors it to the endoplasmic reticulum membrane. As an important member of endoplasmic reticulum calcium-binding proteins, CCDC47 binds calcium ions and participates in regulating calcium store homeostasis within the ER lumen, thereby influencing calcium-dependent signaling and apoptotic processes. Additionally, this protein is involved in ER-associated protein degradation pathways and the formation of ER-mitochondria contact sites, helping to maintain functional coupling between organelles. Genetic studies have revealed that mutations in the CCDC47 gene are directly associated with a rare progressive neurological disorder—early-onset spastic paraplegia with optic atrophy and cognitive impairment (SPOAN syndrome)—highlighting the irreplaceable protective role of this protein in neurodevelopment and myelination processes.

Picture

Western Blot

WB result of CCDC47 Recombinant Rabbit mAb
Primary antibody: CCDC47 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 65 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cerebellum. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CCDC47 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.