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CBLB/RNF56(36-426) Protein, Human

CBLB/RNF56(36-426) Protein, Human

Catalog Number: UA085043 Brand: UA BIOSCIENCE
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$470 USD
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Product Details

Product Specification


Species Human
Synonyms CBL-B, CBLB, RNF56, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56
Accession Q13191
Amino Acid Sequence

Pro36-Phe426
Expression System E.coli
Molecular Weight 40-55kDa (Reducing)
Purity ＞95% by SDS-PAGE & HPLC
Conjugation Unconjugated
Tag No Tag
Physical Appearance Liquid
Storage Buffer

50mM Tris, 200mM NaCl, 1mM DTT, pH7.5, 10%Glycerol
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.
Reference

1. PKC-theta modulates the strength of T cell responses by targeting Cbl-b for ubiquitination and degradation. Science Signaling, 2(76), ra30.
2. Negative regulation of lymphocyte activation and autoimmunity by the molecular adaptor Cbl-b. Nature, 403(6766), 211-216.
3. Cbl-b regulates the CD28 dependence of T-cell activation. Nature, 403(6766), 216-220.

Background

CBLB (Casitas B-lineage Lymphoma Proto-Oncogene B), also known as RNF56 (RING Finger Protein 56), is an E3 ubiquitin-protein ligase that plays a critical role as a negative regulator of immune receptor signaling. It belongs to the CBL family of RING finger E3 ubiquitin ligases and is encoded by the gene located on chromosome 3q13.11.

Mechanism of Action: CBLB promotes proteasome-mediated protein degradation by transferring ubiquitin from specific E2 ubiquitin-conjugating enzymes to substrate proteins. Structurally, it contains several functional domains: an N-terminal tyrosine kinase-binding (TKB) domain (composed of a four-helix bundle, a Ca²⁺-binding EF-hand, and a variant SH2 domain), a proline-rich domain, a nuclear localization signal, a C3HC4-type RING-HC finger, and a ubiquitin-associated (UBA) domain. The RING finger domain confers the E3 ligase activity essential for ubiquitination.

Picture

SDS-PAGE

2μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of CBLB/RNF56(36-426) Protein, Human is more than 95% determined by SEC-HPLC.