WB result of Caspase-2 Recombinant Rabbit mAb
Primary antibody: Caspase-2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Jurkat whole cell lysate 20 µg
Lane 2: Jurkat treated with 25 μM Staurosporine for 5 hours whole cell lysate 20 µg
Lane 3: Molt-4 whole cell lysate 20 µg
Lane 4: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 48, 33 kDa
Caspase-2 Recombinant Rabbit mAb (S-3188-3)
Caspase-2 Recombinant Rabbit mAb (S-3188-3)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Caspase-2 |
| Synonyms | CASP-2; Neural precursor cell expressed developmentally down-regulated protein 2 (NEDD-2); Protease ICH-1; ICH1; NEDD2; CASP2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42575 |
| Clone Number | S-3188-3 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Staurosporine treated Jurkat cells |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
Caspase-2 is an evolutionarily conserved initiator caspase that localizes to the nucleus, cytosol, and Golgi apparatus, where it exists as an inactive 48 kDa pro-enzyme that is activated by dimerization and subsequent autocatalytic processing in response to genotoxic stress, metabolic dysfunction, or mitotic catastrophe, thereby cleaving a limited set of substrates—including MDM2, Bid, and caspase-3—to trigger either a delayed mitochondrial-apoptotic pathway that is independent of p53 or a direct caspase-3 cascade, while also unexpectedly promoting cell survival and genomic stability in some contexts through non-apoptotic functions such as limiting aneuploidy and enhancing DNA repair, illustrating its dual role as both a tumor suppressor and a context-dependent oncogenic regulator.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- Caspase-2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- Caspase-2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- Caspase-2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Caspase-2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Caspase-2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
