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Caspase-12 Recombinant Rabbit mAb

Caspase-12 Recombinant Rabbit mAb

Catalog Number: S0B60271 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Unconjugated Brand: Starter
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Product Details

Product Specification


Host Rabbit
Antigen Caspase-12
Synonyms Caspase-12; Casp12
Location Cytoplasm, Endoplasmic reticulum
Accession O08736
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Ms, Rt
Positive Sample mouse liver, rat liver
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution


application dilution species
WB 1:1000 Ms, Rt
IHC-P 1:500 Ms, Rt

Background

Caspase-12 is a cysteine protease specifically localized to the endoplasmic reticulum (ER) that plays a pivotal role in mediating ER stress-induced apoptosis triggered by stimuli such as calcium overload, hypoxia, and glucose deprivation. It is also closely associated with neurodegenerative processes, as it co-localizes with Alzheimer's disease-related proteins and mediates amyloid-beta-induced cytotoxicity. Notably, while Caspase-12 is fully functional in rodents, the human CASP12 gene contains a premature stop codon in most populations, rendering it a pseudogene with no protease activity.

Picture

Western Blot



WB result of Caspase-12 Recombinant Rabbit mAb
Primary antibody: Caspase-12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 53 kDa

WB result of Caspase-12 Recombinant Rabbit mAb
Primary antibody: Caspase-12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 39 kDa
Observed MW: 52 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat kidney. Anti- Caspase-12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.