WB result of Carbonic Anhydrase 9 Rabbit mAb
Primary antibody: Carbonic Anhydrase 9 Rabbit mAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50 kDa
Observed MW: 54, 58 kDa
Carbonic Anhydrase 9 Recombinant Rabbit mAb (SDT-733-28)
Carbonic Anhydrase 9 Recombinant Rabbit mAb (SDT-733-28)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Carbonate dehydratase IX, Carbonic anhydrase IX (CA-IX; CAIX), Membrane antigen MN, P54/58N, Renal cell carcinoma-associated antigen G250 (RCC-associated antigen G250), pMW1, CA9, G250, MN |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q16790 |
| Clone Number | SDT-733-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 |
Background
Carbonic anhydrase IX (CA9/CAIX) is an enzyme encoded by the CA9 gene. It is one of the 14 subtypes of carbonic anhydrase discovered by humans, a transmembrane dimer metalloenzyme with extracellular active sites that promote gastrointestinal acid secretion. CAIX is overexpressed in many types of cancers, including clear cell renal cell carcinoma (RCC), cervical cancer, breast cancer, and lung cancer. In these cancers, CAIX promotes tumor growth by enhancing tumor acidosis.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti- Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human renal clear cell cancer. Anti-Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human chromophobe renal carcinoma. Anti- Carbonic Anhydrase 9 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
