WB result of CAPRIN1 Mouse mAb
Primary antibody: CAPRIN1 Mouse mAb at 1/5000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 116 kDa
CAPRIN1 Mouse mAb
CAPRIN1 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CAPRIN1 |
| Synonyms | Caprin-1; Cell cycle-associated protein 1; Cytoplasmic activation- and proliferation-associated protein 1; GPI-anchored membrane protein 1; GPI-anchored protein p137 (GPI-p137; p137GPI); Membrane component chromosome 11 surface marker 1; RNA granule protein 105; GPIAP1; GPIP137; M11S1; RNG105 |
| Location | Cytoplasm |
| Accession | Q14444 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1 |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:10000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:100 | Hu, Ms |
Background
CAPRIN1 (Cell Cycle Associated Protein 1) is a multifunctional RNA-binding protein that plays critical roles in regulating cell proliferation, stress response, and innate immunity by modulating mRNA stability, translation, and signal transduction pathways. Primarily localized in cytoplasmic ribonucleoprotein granules known as stress granules, CAPRIN1 facilitates the assembly of these membraneless organelles under cellular stress conditions to temporarily repress translation and protect mRNAs from degradation, while also interacting with key signaling molecules like G3BP1 to influence cell cycle progression and apoptosis. Beyond its role in stress adaptation, CAPRIN1 has been implicated in tumorigenesis and viral defense mechanisms, as it can either promote or suppress cancer cell growth depending on context and serves as a regulator of antiviral responses by interacting with proteins involved in interferon signaling, making it a significant biomarker and potential therapeutic target in oncology and infectious disease research.
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Picture
Western Blot
WB result of CAPRIN1 Mouse mAb
Primary antibody: CAPRIN1 Mouse mAb at 1/5000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 116 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CAPRIN1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-CAPRIN1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-CAPRIN1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-CAPRIN1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-CAPRIN1 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
