CAP1 Rabbit Polyclonal Antibody

Catalog Number: S0B6990 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CAP1
Synonyms	Adenylyl cyclase-associated protein 1; CAP
Immunogen	Synthetic Peptide
Location	Cell membrane
Accession	Q01518
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	293T, HeLa, MCF7, L-929, mouse lung, rat lung, COS-7
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt, Mk
IHC-P	1:1000	Hu

Background

CAP1, also known as Adenylate Cyclase-Associated Protein 1, is a highly conserved and multifunctional actin-regulatory protein that plays a pivotal role in cytoskeletal dynamics by coordinating actin monomer recycling and filament turnover. Structurally, CAP1 functions as a hexameric complex that bridges the activities of ADF/cofilin and profilin; it accelerates the dissociation of cofilin from ADP-actin monomers, facilitates the exchange of ADP for ATP on these monomers, and subsequently delivers the ATP-actin to profilin for efficient re-polymerization at the barbed ends of filaments. Beyond its canonical function in maintaining the global pool of polymerization-competent actin, CAP1 is implicated in various cellular processes including cell migration, endocytosis, and signal transduction pathways involving cyclic AMP, with dysregulation or mutations in the CAP1 gene being associated with developmental defects and immune disorders in humans, thereby highlighting its essential contribution to cellular architecture and physiological homeostasis.

Picture

Western Blot

WB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDa

WB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: L-929 whole cell lysate 20 µg
Lane 2: mouse lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDa

WB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDa

WB result of CAP1 Rabbit pAb
Primary antibody: CAP1 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 54 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human testis. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CAP1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.