1 1

Canine Pancreatic lipase, His tag

Canine Pancreatic lipase, His tag

Catalog Number: S0A9047 Brand: Starter
Share:
Price:
$310 USD
$310 USD
Size:

For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Species Canine
Synonyms Pancreatic lipase
Accession A0A8I3QQI4
Amino Acid Sequence

Protein sequence (A0A8I3QQI4, Lys17-Cys465, with C-10*His)KEVCFPRLGCFSDDSPWAGIVERPLKILPWAPKDVNTRLLLYTNENPDNFQELTADPSIITSSNFKTDRKTRFIIHGFIDKGEESWLANMCKKMFVVESVNCICVDWKSGSRTGYTQASQNIRIVGAEVAYFVEVLQSAFGYSPSDVHIIGHSLGAHAAGEAGRRLNGTAGRITGLDPAEPCFEGTPELVRLDPSDAQFVDVIHTDAAPIIPNMGFGMSQTVGHLDFFPNGGKEMPGCQKNILSQIVDIDGIWEGTRDFVACNHLRSYKYYSDSILNPDGFAGFPCASYNVFTANKCFPCPSEGCPQMGHYADRFPGKTDKVNQIFYLDTGDASNFARWRYKVAVTLSGKKVTGHVLVSLFGNKGNSKQYEIFKGTLQPESTHSNEFDSDVEVGDVQKVKFVWYNNVINPTLPRVGASKITVERNDGKIFNFCSKETVREDILLTLTPCGGGGSHHHHHHHHHH
Expression System HEK293
Molecular Weight Predicted MW: 51.4 kDaObserved MW: 56 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Tag with C-His Tag
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Pancreatic lipase, also known as pancreatic triacylglycerol lipase or steapsin, is an enzyme secreted from the pancreas. As the primary lipase enzyme that hydrolyzes (breaks down) dietary fat molecules in the digestive system, it converting triglyceride substrates like 1 found in ingested oils to monoglycerides 3 and free fatty acids 2a and 2b. Pancreatic lipase is secreted in its final form. It becomes efficient only in the presence of colipase in the duodenum. Its concentration in serum is normally very low. Under extreme disruption of pancreatic function, such as pancreatitis or pancreatic adenocarcinoma, the pancreas may begin to autolyse and release pancreatic enzymes including pancreatic lipase into serum. Thus, through measurement of serum concentration of pancreatic lipase, acute pancreatitis can be diagnosed.

Picture

SDS-PAGE

2μg（R: reducing conditions; NR: non-reducing conditions）