WB result of Calreticulin Recombinant Rabbit mAb
Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 58 kDa
Calreticulin Recombinant Rabbit mAb (S-3920-39)
Calreticulin Recombinant Rabbit mAb (S-3920-39)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Calreticulin |
| Synonyms | CRP55; Calregulin; Endoplasmic reticulum resident protein 60 (ERp60); HACBP; grp60; CRTC; CALR |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Endoplasmic reticulum lumen |
| Accession | P27797 |
| Clone Number | S-3920-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HL-60, HepG2, NIH/3T3, RAW264.7, Neuro-2a, C2C12, mouse heart, mouse brain, mouse colon, mouse stomach, mouse kidney, mouse liver, rat heart, rat brain, rat colon, rat stomach, rat kidney, rat liver |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2500 | Hu, Ms, Rt |
| ICC | 1:250 | Ms |
Background
Calreticulin (CRT) is a highly conserved, multifunctional calcium-binding chaperone protein primarily residing in the lumen of the endoplasmic reticulumER), where it plays a pivotal role in maintaining cellular calcium homeostasis and facilitating the proper folding and quality control of newly synthesized glycopptides through its interaction with the calnexin/calreticulin cycle. Beyond its canonical intracellular functions, CRT exhibits significant "moonlighting" properties by translocating to the cell surface under stress conditions, such as immunogenic cell death, where it acts as a critical "eat-me" signal that promotes the phagocytosis of apoptotic or dying cells by dendritic cells and macrophages via binding to the LDL receptor-related protein 1 (LRP1/CD91), thereby bridging innate and adaptive immunity. Furthermore, extracellular CRT has been implicated in various pathological processes, including wound healing, angiogenesis, and autoimmune diseases like systemic lupus erythematosus, making it a subject of intense research for potential therapeutic interventions in cancer immunotherapy and inflammatory disorders.
Picture
Picture
Western Blot
WB result of Calreticulin Recombinant Rabbit mAb
Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: Neuro-2a whole cell lysate 20 µg
Lane 4: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 58 kDa
WB result of Calreticulin Recombinant Rabbit mAb
Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Lane 3: mouse colon lysate 20 µg
Lane 4: mouse stomach lysate 20 µg
Lane 5: mouse kidney lysate 20 µg
Lane 6: mouse liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 58 kDa
WB result of Calreticulin Recombinant Rabbit mAb
Primary antibody: Calreticulin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat colon lysate 20 µg
Lane 4: rat stomach lysate 20 µg
Lane 5: rat kidney lysate 20 µg
Lane 6: rat liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 58 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Calreticulin antibody was used at 1/2500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti-Calreticulin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
