Calpain 1 Recombinant Rabbit mAb (S-3797-41)

Catalog Number: S0B6989 Application: WB, IHC-P Reactivity: Hu, Ms, Rt, Mk Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Calpain 1
Synonyms	Calpain-1 catalytic subunit; Calcium-activated neutral proteinase 1 (CANP 1); Calpain mu-type; Calpain-1 large subunit; Cell proliferation-inducing gene 30 protein; Micromolar-calpain (muCANP); CAPN1; CANPL1
Immunogen	Synthetic Peptide
Location	Cytoplasm, Cell membrane
Accession	P07384
Clone Number	S-4115-48
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt, Mk
Positive Sample	293T, A431, Jurkat, NIH/3T3, mouse brain, C6, rat brain, COS-7
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms, Rt, Mk
IHC-P	1:200	Hu, Ms, Rt

Background

Calpain 1 (CAPN1), also known as μ-calpain, is a ubiquitously expressed, calcium-dependent non-lysosomal cysteine protease in humans. It typically exists as a heterodimer consisting of an 80 kDa catalytic large subunit encoded by the CAPN1 gene and a 28 kDa regulatory small subunit encoded by the CAPNS1 gene. Calpain 1 is uniquely activated by micromolar levels of calcium ions, hence its name μ-calpain (micro-calpain), distinguishing it from its homolog, calpain 2, which requires millimolar calcium concentrations for activation. Under physiological conditions, Calpain 1 is localized to various cellular compartments, including the cytoplasm and mitochondria. It performs limited proteolytic cleavage of specific substrates and participates in key processes such as cytoskeletal remodeling, signal transduction, and cell migration. However, under pathological conditions such as ischemia-reperfusion injury and Alzheimer's disease, disruption of intracellular calcium homeostasis can lead to excessive activation of Calpain 1, resulting in aberrant cleavage of various functional proteins (e.g., apoptosis-inducing factor AIF and junctophilin-2 in the heart), thereby mediating cellular damage and dysfunction. Therefore, precise regulation of Calpain 1 is essential for maintaining cellular health and function, making it a potential therapeutic target for various diseases.

Picture

Western Blot

WB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa

WB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa

WB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa

WB result of Calpain 1 Recombinant Rabbit mAb
Primary antibody: Calpain 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 80 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat colon. Anti-Calpain 1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.