WB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
CALHM2 Recombinant Rabbit mAb (S-2671-146)
CALHM2 Recombinant Rabbit mAb (S-2671-146)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CALHM2 |
| Synonyms | Calcium homeostasis modulator protein 2; Protein FAM26B; FAM26B |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9HA72 |
| Clone Number | S-2671-146 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | U-2 OS, HeLa, U-87 MG, NIH/3T3, COS-7 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Mk |
| IHC-P | 1:50 | Hu |
| ICC | 1:50 | Hu |
Background
CALHM2 (Calcium Homeostasis Modulator 2) is a 323-amino-acid, four-pass transmembrane protein that oligomerizes into an undecameric, voltage-gated channel which—in addition to its originally described roles in taste-bud ATP release and neuronal excitability—has recently been shown to reside in the inner mitochondrial membrane where it functions as a Ca²⁺-inhibited import channel that translocates the ECHA subunit of the mitochondrial trifunctional protein into the matrix to enable fatty-acid β-oxidation, cristae integrity and normal respiratory metabolism, thereby coupling ion-channel gating to cellular energy supply and implicating CALHM2 in metabolic and neuropsychiatric disorders.
Picture
Picture
Western Blot
WB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
WB result of CALHM2 Recombinant Rabbit mAb
Primary antibody: CALHM2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 36 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CALHM2 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U2-OS cells. Anti-CALHM2 antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
