CAIX Recombinant Rabbit mAb (SDT-356-21)

Catalog Number: S0B0109 Application: WB, IHC-P Reactivity: Ms, Rt Conjugation: Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	CAIX
Synonyms	CA9, Carbonic anhydrase 9, Carbonate dehydratase IX, Membrane antigen MN homolog
Immunogen	Recombinant Protein
Location	Cytoplasm, Cell membrane
Accession	Q8VHB5
Clone Number	SDT-356-21
Antibody Type	Recombinant mAb
Application	WB, IHC-P
Reactivity	Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Tag	N/A
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Ms
IHC-P	1:8000	Ms, Rt

Background

Carbonic anhydrase IX has been under intensive investigation as a therapeutic targetin cancer. Studies demonstrate that this enzyme has a key role in pH regulation in cancer cells,allowing these cells to adapt to the adverse conditions of the tumour microenviroment. NovelCAIX inhibitors have shown efficacy in both in vitro and in vivo pre-clinical cancer models, adverselyaffecting cell viability, tumour formation, migration, invasion, and metastatic growth when usedalone. In co-treatments, CAIX inhibitors may enhance the effects of anti-angiogenic drugs orchemotherapy agents [PMID: 29439394].

Picture

Western Blot

WB result of CAIX Rabbit mAb

Primary antibody: CAIX Rabbit mAb at 1/1000 dilution

Lane 1: mouse stomach lysate 20 µg

Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution

Predicted MW: 50kDa

Observed MW: 54kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse liver. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat stomach. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded rat kidney. Anti-CAIX antibody was used at 1/8000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.