WB result of C3 Recombinant Rabbit mAb
Primary antibody: P C3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Lane 2: human plasma lysate 5 µg
Lane 3: mouse serum lysate 20 µg
Lane 4: mouse plasma lysate 20 µg
Lane 5: rat serum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG(H+L) Recombinant pAb (min X Hu Sr Prot), HRP conjugated at 1/10000 dilution
Predicted MW: 187 kDa
Observed MW: 38, 70, 115, 187 kDa
C3 Recombinant Rabbit mAb (S-5146)
C3 Recombinant Rabbit mAb (S-5146)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | C3 |
| Synonyms | Complement C3; C3adesArg; More chains; CPAMD1 |
| Location | Secreted |
| Accession | P01024 |
| Clone Number | S-5146 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | human serum, human plasma, mouse serum, mouse plasma, rat serum |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:500-1:2000 | Hu, Ms, Rt |
| IF | 1:500 | Hu, Ms, Rt |
Background
C3 plays a pivotal role in initiating both the classical and alternative pathways of the complement system. Upon activation by C3 convertases, C3 is cleaved into C3a and C3b fragments. C3b covalently binds to cell surface carbohydrates or immune aggregates, while C3a acts as an anaphylatoxin mediating local inflammatory responses. Beyond inflammation, C3 regulates the intensity and direction of immune responses through interactions with other complement components. Detection of C3 levels is valuable for diagnosing, monitoring, and assessing prognosis in various diseases, such as autoimmune disorders, infectious diseases, and cardiovascular conditions. As a crucial component of the complement system, C3 is a significant target for drug development and immunotherapy research.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-C3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human placenta. Anti-C3 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-C3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-C3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-C3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human liver. Anti-C3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded mouse liver. Anti-C3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat liver. Anti-C3 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
