The BTK[T474S] activity was detected using HTRF technology. The reaction was performed by incubating the BTK[T474S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
BTK[T474S] Protein
BTK[T474S] Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | Q06187-1 |
| Amino Acid Sequence | A2-S659(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 101876.0 |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Hepes, 150mM NaCl, 20mM Reduced Glu, 1mM DTT, 10% Glycerol, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg BTK[T474S]
2 3 μg BTK[T474S]
