IHC shows positive staining in paraffin-embedded human osteosarcoma. Anti-BSP II antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
BSP II Recombinant Rabbit mAb,PBS Only (SDT-3508-1)
BSP II Recombinant Rabbit mAb,PBS Only (SDT-3508-1)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BSP II |
| Synonyms | Integrin-binding sialoprotein; Bone sialoprotein 2; Bone sialoprotein II (BSP II); Cell-binding sialoprotein; BNSP; IBSP |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P21815 |
| Clone Number | SDT-3508-1 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| IF | 1:2000 | Ms, Rt |
Background
BSP II (Bone Sialoprotein II) is a heavily sialylated, secreted glycoprotein that constitutes up to 10 % of bone’s non-collagenous matrix, where its clusters of acidic amino acids confer high-affinity binding to hydroxyapatite, its poly-Glu sequences nucleate mineral deposition, and its C-terminal RGD motif supports osteoblastic and odontoblastic adhesion via αvβ3 integrin, thereby acting as a key structural and signaling scaffold that synchronizes the onset of matrix mineralization with cell attachment during skeletal and cementum formation.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse E14.5 embryo. Anti-BSP II antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse E14.5 embryo (enlarged detail). Anti-BSP II antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat E14.5 embryo. Anti-BSP II antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat E14.5 embryo (enlarged detail). Anti-BSP II antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse E14.5 embryo. Anti-BSP II antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
IF shows positive staining in paraffin-embedded rat E14 embryo. Anti-BSP II antibody was used at 1/4000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
